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Rational for discussing cardiorespiratory fitness, zone 2, and VO max despite having covered the topic extensively [2:30]

Laboratory assays repeatedly show it stimulating fibroblast-driven synthesis of collagen and elastin

Cells were cultured in Dulbeccos Modified Eagles medium (Welgene LM001-05) supplemented with 10% fetal bovine serum (Gibco 16000-044), and 1% penicillin/streptomycin (Gibco 15140-122)

Activation of calmodulin-dependent NAD+ kinase by trypsin [PMID: 6279162] Laboratory literature summary: Activation of calmodulin-dependent NAD+ kinase by trypsin

The process takes about 30 seconds and is virtually painless