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Krka, Slovenia) intraperitoneally), we induced abdominal compartment syndrome as described before and maintained high abdominal pressure at 25 mmHg for 120 min before sacrifice

Remarkably, both parameters were dramatically enhanced when mice were preinjected with the ferroptosis inhibitor ferrostatin-1 (Fer-1) (Figure 1A-C)

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Western blotting Briefly, 0.5 ml of RIPA lysis buffer was used to prepare a tissue lysate from the mouse prostate, along with the addition of 0.1 ml of proteinase inhibitor mixture for mechanical homogenization

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