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In the Eqn.4 L is the concentration of the competing ligand, K 1 and K 2 correspond to the dissociation constants of CuL and CuL 2 complexes, respectively [Cu] total is the concentration of copper and [Cu] bound to protein is the concentration of the copper bound to the protein at IC50
The name comes from exactly where you think it does
The area percentage of the main peak is the purity figure
some peptide reaches systemic circulation (supported by systemic effect reports) but at likely reduced bioavailability compared to parenteral
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