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Substance use disorder is either directly or indirectly responsible for 11.8 million annual deaths
This resulted in hypercalcemia and hypokalemia, which affected the propagation of electrical impulses and depolarization in the heart 71
In either situation, the cells were then permeabilized with cold PBST (0.5% Triton X-100) for 10 min, followed by fixation with 4% paraformaldehyde (PFA) in PBS (pH 7.4) for 15 min at room temperature
Repeat daily as indicated
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