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Mice were maintained in a temperature-controlled room (2122 C) on a 12 hr light/dark cycle and supplied with food and water ad libitum

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This multi-axis neuroendocrine regulatory profile positions DSIP as a broad hypothalamic-pituitary axis modulator, not merely a sleep-specific peptide, and makes it a research tool for studying the neuroendocrine consequences of altered sleep homeostasis and circadian rhythm disruption