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[125] In 2016 Imai expanded his hypothesis to "NAD World 2.0", which postulates that extracellular NAMPT from adipose tissue maintains NAD + in the hypothalamus (the control center) in conjunction with myokines from skeletal muscle cells
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After validating their mesenchymal identity, hGMSCs were cultured to the third passage and then divided into two groups: one treated with 0.01 g/mL of Epitalon in PBS, and a control group without treatment
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