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(4) Add 300 L of chloroform-methanol (V:V=2:1) into the centrifugal tube with the lower layer of solution, vortex for 30 s, and ultrasonic extraction for 10 min in an ice-water bath
Redman LM, Smith SR, Burton JH et al (2018) Metabolic slowing and reduced oxidative damage with sustained caloric restriction support the rate of living and oxidative damage theories of aging
In a healthy system, intracellular glutathione immediately neutralizes these free radicals, protecting the delicate mitochondrial membranes from lipid peroxidation and structural damage
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