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Without lab-guided dosing, you are guessing with hormonal signaling molecules
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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)
Vials are cushioned inside a rigid mailer, sealed with tamper-evident tape, and packed in discreet outer packaging with no product name visible
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