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The addition of murine culture media with GSH increased the freezing tolerance of embryos by reducing ROS levels, inhibiting apoptotic, and improving oxidative status []

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EE induced C2C12 myotube hypertrophy was blocked by inhibitors of IGF-1 signal pathway Inhibitors of IGF-1 signal pathway, such as LY294002 (PI3K inhibitor) and rapamycin (mTOR inhibitor), were applied 30 min prior to EE, IGF-1, and testosterone treatment to evaluate the influence of their effects on myotube hypertrophy and the expression levels of MyHC