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Archived from the original on 4 March 2016
The metabolic effects of metformin vary somewhat across gram-negative bacteria: (1) in E coli metformin inhibits respiratory chain complex I, impairing the function of the inner membrane vitamin B12 transporter (BtuCD), so vitamin B12 entry is blocked at the inner membrane, causing vitamin B12 to accumulate in the periplasm
Tosoh, Tokyo Japan) and electrospray ionization time-of-flight mass spectrometry with a Hitachi Nano Frontier liquid chromatographymass spectrometry (LC-MS) system (Tokyo, Japan)
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