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A machine learning approach for predicting CRISPR-Cas9 cleavage efficiencies and patterns underlying its mechanism of action

Research has focused on its ability to influence glucose metabolism, insulin sensitivity, and mitochondrial function , making it a key subject in studies on metabolic health and aging

GCL is specific for the glutamyl moiety and is regulated physiologically by: (a) non-allosteric feedback competitive inhibition by GSH (Ki=2.3mM), which involves binding of GSH to the glutamate and another site on the enzyme [8, 9] and (b) availability of its precursor, cysteine [1] .The apparent Km values of GCL for glutamate is 1.8 mM, and for cysteine and 0.10.3 mM [9]

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