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We also explored several other known regulators of PGC1 activity that are altered in response to 5FU treatment in HCT116, including NRF2, reactive oxygen species and PPAR, but none of these prevented PGC1-driven increases in mitochondrial mass

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The broad-spectrum thioesterase inhibitor palmostatin B also has off-target effects and poor drug specificity, leading to a lack of stability, thus limiting its clinical use (249, 250)