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Peptides (prepared as 5 solution in HBSS and 20 mM HEPES, pH 7.4) were added at time=10 s and changes in fluorescence were monitored over a period of 250 s following excitation at a wavelength of 488 nm and detection at 510560 nm

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Lancet 359:20532058 Belch J, Hiatt WR, Baumgartner I, Driver IV, Nikol S, Norgren L, Van Belle E, Committees T, Investigators (2011) Effect of fibroblast growth factor NV1FGF on amputation and death: a randomised placebo-controlled trial of gene therapy in critical limb ischaemia