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This makes it loosely bound to Insulin-like Growth Factor Binding Proteins (IGFBPs)
The N-terminal segment (amino acids 1200, E3-N) and C-terminal segment (amino acids 201-658, E3-C) of E3 were inserted into the bait vector pGBKT7 to generate pGBKT7-E3-N, pGBKT7-E3-C, respectively
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