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Cancer cells were washed with PBS plus 0.02% Triton X-100 (Sigma-Aldrich, Dorset, UK) 3 times for 5 min each and blocked with 10% donkey serum (Sigma-Aldrich, Dorset, UK) for 30 min
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9i), suggesting that cotargeting GLUT1 and YBX1 confers selective synthetic lethality and has a potentially broad therapeutic effect
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Future work will use longitudinal profiling and single-cell sequencing to assess cell-type-specific GSH roles 5 Conclusion In conclusion, by leveraging multi-omics integration, the present study identified GSH metabolism as a pivotal node in SAE, while providing a comprehensive molecular framework for SAE pathogenesis, and linking microglia-driven neuroinflammation to hippocampal oxidative damage
