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Site-directed mutagenesis Mutant forms of mito GCL refractory to the shRNA against GCL ( mito GCLmut) or catalytically inactive mito GCL ( mito GCL (E103A)) were generated using the site-directed mutagenesis QuikChange XL kit (Stratagene, La Jolla, CA, USA), followed by Dpn I digestion
Synthesis of IL-2 mRNA in Cells of Rat Hypothalamic Structures after Injection of Short Peptides
For instance, there was an initial decrease followed by a dramatic increase in beta-endorphin
Presented for research literature review only Smith JE (1973)
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