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While the largest percentage of intracellular iron is tightly bound to or incorporated into proteins as a cofactor or for storage, small portion of intracellular iron resides in the cytosol and intracellular organelles (e.g., lysosomes) and constitutes a redox-active liable iron pool, which regulates programmed cell death including ferroptosis 42

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The use of total effect indices negates the need for determining higher order interactions which can be computationally expensive

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