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For Western blotting and immunofluorescence staining, the following antibodies were used: LOXL2 rabbit monoclonal (Abcam, ab179810), LOX rabbit polyclonal (Invitrogen, PA1-46020), LOXL1 mouse monoclonal (Santa Cruz, sc-166632), LOXL3 rabbit polyclonal (Santa Cruz, sc-68939), VAP-1 (AOC3) mouse monoclonal antibody (Santa Cruz, sc-373924), Collagen I monoclonal antibody (Invitrogen, MA1-26771), Collagen IV polyclonal antibody (Assay Biotech, C0157), GAPDH mouse monoclonal (Novus Bio, NB300221), goat anti-mouse IgG (H + L)-HRP conjugate (Biorad 1706516), AffiniPure goat anti-rabbit IgG (H + L)-HRP conjugate (Jackson ImmunoResearch, 111035144), Cy5 AffiniPure Goat Anti-Rabbit IgG (H + L) (Jackson ImmunoResearch, 111175144), Alexa Fluor 568 Goat anti-Rabbit IgG (H + L) Secondary Antibody (Invitrogen, A-11011), Alexa Fluor 488 Donkey anti-Mouse IgG (H + L) Secondary Antibody (Invitrogen, A-21202) and fluorescein (DTAF) streptavidin (Jackson ImmunoResearch, 016010084)
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This is important because when we're talking about peptide therapeutics
At optimal levels, it maintains healthy skin remodeling
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