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In brief, the N-terminal and transmembrane region of one receptor and the C-terminal tail of the other receptor were amplified with primers that introduced a HindIII restriction site directly upstream of the start codon of the receptor and replaced the stop codon with an XbaI restriction site (primers are shown in Supplementary Table 1)
10.1371/journal.pone.0166125 180 TahraniA
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