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In plants, the first step of glutathione biosynthesis takes place in plastids, and the second step in the cytosol
Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development
however, it too has been withdrawn from the market due to an association with bowel ischemia and for possible cardiovascular side effects
