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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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Our study, building upon the MMPM model, introduced an HFD + ADF regimen to alter the type of food intake and normal dietary rhythm of rats, thereby intensifying the impact of dietary factors

GHRH-Like Action: Stimulates growth hormone secretion in laboratory models