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Annexin V binds phosphatidylserines that translocate to the outer leaflet of the membrane during early apoptosis 61 , whereas 7-AAD is a membrane-impermeable DNA dye that labels cells with compromised membrane integrity, identifying cells undergoing lytic forms of cell death such as necrosis, secondary necrosis and pyroptosis 62,63

Therefore, monitor these two, and you may want to increase your potassium intake in the first few months of injecting

84: 25765 Meyers C, Bonow R, Palmeri S, et al

I was purchasing B-12 filled needles exact dose from my vet prior to this

It surfaces the parameters the supervised protocol uses