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After protein quantification, loading buffer containing 10% SDS, 0.5% bromophenol blue in Tris-HCL (pH 6.8), and 10% -mercaptoethanol (M3148, Sigma) was added to each cell lysate and boiled at 95-100 C for 10 min
Cantorna MT, Snyder L, Arora J
Batch specificity: The CoA should correspond to the specific lot number of the vials you receive, not a generic document applicable to any batch
CPT2 activity CPT2 activity in cell lysates was determined by using commercial CPT2 activity colorimetric quantitative detection kits (Shenzhen Ziker Biological Technology Co., Ltd., Shenzhen, China), according to the manufacturers instruction
One study showed that adults who slept fewer than six hours each night were more likely to catch a cold or the flu than those who slept more than six hours each night
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