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Subsequently, slides were washed 3X with PBS (10 min, RT) and incubated with the secondary horseradish peroxidase conjugated antibody (mouse anti-rabbit IgG, 0.4 mg/mL diluted 1:500, sc-2357 or goat anti-mouse IgG, 0.4 mg/mL diluted 1:500, sc-2031) for 2 h at RT, followed by three PBS washes and treated with the 3,3-diaminobenzidine substrate (ab64238, Abcam) for 10 min
Furthermore, the results suggest that the homocysteinylation might be regulated by the thiol redox status
doi: 10.1038/ijo.2014.153 73 StratiFCavalieriDAlbaneseDDe FeliceCDonatiCHayekJet al
The total volume also plays a role
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