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We then used a Cryostat Microtome CM3050S (Leica, Germany) to section the muscle sample, the tissue sections were stored at 80 C for later Hematoxylin and Eosin stain (H&E stain), Gmri trichrome stain, and nicotinamide adenine dinucleotide dehydrogenasetetrazolium reductase (NADH-TR) stain as described previously 28
Endpoints range from EEG-derived sleep-stage proportions in restrained and unrestrained animals to plasma hormone panels, behavioral pain assays and opioid-interaction protocols
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Tc l 500mg Gltathione tng ng 1000mg vitamic C
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