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Biosci Rep (2019) 39(2):BSR20181995
Stimulation with 6-hydroxydopamine (6-OHDA) markedly downregulates nuclear factor erythroid 2related factor 2 (NRF2) expression in PC-12 cells, elevates Fe 2+ and reactive oxygen species (ROS) levels, suppresses glutathione peroxidase 4 (GPX4), promotes both ferroptosis and apoptosis, inhibits cell proliferation, and causes neuronal damage
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The resulting single-stranded cDNAs were used as templates in PCR reactions using gene-specific primers to amplify TGR (forward 5-CTATTTCCGTAGACGTCTGT-3 and reverse 5-AATACAGTTTCCTTCCCGTT-3) or GAPDH (forward 5-GTTTTGGTCGTATCGGGAGA-3 and reverse 5-ATGCGTTAGAAACCACGGAC-3)
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