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LC-MS/MS is widely regarded as a gold standard due to its inherent sensitivity, specificity, and capability for simultaneous quantification of multiple vitamins and their metabolites from limited sample volumes, which is particularly advantageous in pediatric CF patients ( 6.3 Assessment of total antioxidant capacity (TAC) CF pathophysiology involves disrupted cellular equilibrium alongside persistent inflammatory responses that drive progressive tissue deterioration, with particular severity in pulmonary manifestations ( Multiple analytical techniques utilizing spectrophotometric and fluorometric detection have emerged for TAC quantification in biological specimens, with each methodology employing unique chemical mechanisms to evaluate integrated antioxidative responses
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