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Outflow fractions were collected every 10 minutes and measured using a glucose/glucose oxidase kit in which lactate oxidase was used to replace glucose oxidase
Initial reaction velocities were calculated from the slope of the A 340nm data from 1 min to 3 min using = 9,600 cm 1 M 1 for the concentration calculation of the conjugation product
I.LeeH-J.ChoS
Diabetologia 52(2):199207
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