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Nitrous oxide (laughing gas) exposure

Numerous clinical studies comparing the gut microbiomes of patients with depression to those of healthy controls have revealed significant differences in microbiome composition

Pellet was resuspended in glucose-free RPMI medium supplemented with 2-NBDG (Thermo Fisher N13195) at 50 M and incubated for 30 min at 37 C

Clean vial stoppers with alcohol, add water slowly to avoid damaging the powder, and gently rotate the vial to dissolve it

This shows that care must be taken in interpreting blood measurements, and that ideally one would like to conduct experiments in which both intracellular and extracellular concentrations are measured