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Polysome profiling Cells were lysed in 500 L polysome lysis buffer (25 mM HEPES pH 7.5, 5 mM MgCl 2 , 0.1 M KCl, 2 mM DTT, 1% Triton-X, 0.1 mg/mL cycloheximide) and incubated on ice for 5 min
The months of your life you spend on the protocol are the expensive part, and they deserve a vial you can trust
To interrogate this, we utilized an AAV-TBG-Cre viral delivery system, which produces liver-specific Cre expression and subsequent genetic deletion 49,50
The seal in the Hydrojelly lid is designed to compress to keep the powder inside the container during storage and shipping
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