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The lysed tissue was centrifuged (13000 rpm, 3 min, 4 C) and the supernatant was transferred to a clean Eppendorf tube (low-bind protein) prior to protein concentration determination with the DC Protein Assay (Bio-Rad) according to manufacturers instruction
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Who Considers Epithalon and Why Epithalon is not a mainstream pharmaceutical
Physiological Reviews, 1983
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