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This includes both reduced and oxidized states together, is the most potent endogenous antioxidant, is correlated well with muscle and heart tissue at rest (r = 0.93 to 1.0) [24] and at exercise (r = 0.66 to 1.0) [24], and the assay is designed to move all to the reduced form of measurement so GSH becomes total reduced GSH content in whole blood (ie., GSH+ GSSG + GSSProt)

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Mass spectrometry data confirming correct molecular identity offers additional verification

This necessity limits accessibility and increases the cost, making it less feasible for continuous use