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When it comes to coffee enemas, in particular, its important to talk to your doctor before taking one at home

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Zeratsky, K., & Mayo Clinic

That date assumes you dissolve the B12 in saline as soon as you receive the vial, whether you choose to store it in the fridge or a cool cabinet

Glucose/galactose adapted cells were cultured in DMEM containing 10% dFBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, 1 mM sodium pyruvate and 25 mM glucose (Sigma-Aldrich, G7021-1KG) or 25 mM galactose (Sigma-Aldrich, G5388-500G) for a minimum of 1 month before being used experimentally