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In addition to p38 MAPK and ERK, c-KIT activation is associated with the phosphoinositide 3-kinase (PI3K) signaling pathway, which not only regulates cell survival but also causes pigmentation by activating the serine/threonine-specific protein kinase AKT

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This final suspension was aliquoted into 1ml vials and stored at -20C for 1 hour and then stored to -80C until used for in vitro granulomas formation and infection studies