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After pre-conditioning, cells were washed and incubated in glucose-free DMEM medium containing 20 M 2-NBDG for 30 min at 37 C

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Factors that improve skin elasticity recovery include staying at a stable weight (weight cycling worsens sagging), maintaining adequate protein intake to support collagen synthesis, and avoiding smoking or excessive sun exposure

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Recent developments in the detection and treatment of vitamin B12 deficiency in sheep and cattle at pasture