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Proteins (desalted in 20 mM Tris-HCl buffer) were incubated in buffers with relatively high ionic strength to prevent electrostatic influences on thiolate reactivities and different pH values containing 1 mM of IAM for 1.5 minutes and the reaction was stopped by addition of trichloroacetic acid to 10%
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Reynolds, J.C., Lai, R.W., Woodhead, J.S.T., et al
These are the types of studies that augment our approach to regenerative orthopedics
