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Serum leptin and active ghrelin concentrations were measured using commercial radioimmunoassay (RIA) kits, following the manufacturers instructions

Fish were then incubated overnight at 4C in anti-Met in PBST with 1% goat serum

BPC-157 (Body Protection Compound-157) is a synthetic 15-amino-acid peptide derived from a gastric protein sequence studied for tissue-healing and cytoprotective properties [1] [2]

For hematocrit and reticulocyte counts, recommendations are to repeat these tests daily from the 5th to 7th days of treatment and then frequently until the hematocrit returns to a normal range (11, 14, 15)

An in vitro protocol to study the effect of hyperglycemia on intracellular redox signaling in human retinal pigment epithelial (ARPE-19) cells