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Cells were seeded on low-attachment culture vessels (Corning) and cultured in mTeSR1 medium supplemented with 40 g ml 1 Matrigel, 1 ng ml 1 BMP4 (Invitrogen) and 10 M Rho kinase inhibitor (ROCK) (R&D) under hypoxic environment with 5% O 2
Vargas, L., Coleman, J., & Huang, Y
Psychological comorbidities, such as body dysmorphic disorder, are presumed to be common in Melanotan users
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The study group was administered a 15 g/bag of L-carnitine complex nutrient treatment, orally, twice a day
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