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The diagnosis of T2DM was based on the criteria of the World Health Organization (WHO 1999) [24]
When systemic glutathione levels are low, the resulting unchecked oxidative stress acts as a constant irritant to mast cells, keeping them in a hyper-reactive state
Refrigeration is recommended to maintain the stability of the peptide, but avoid freezing it
The temperature was kept at 300 K in all simulations using a velocity rescaling thermostat 36 , and the pressure was kept at 1 bar (using a Berendsen barostat 37 for the equilibration steps and a ParrinelloRahman barostat 38 for the production)
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