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At day 5 aggregates were gently collected, sieved through a reversible strainer and transferred into matrigel-coated plates with neural proliferation medium

Following seven consecutive days of treatment with fluoxetine, the serotonin content in growing germinal vesicle oocytes and ovulation-matured MII oocytes was significantly decreased, and the expression and activity of SERT were reduced, leading to impaired oocyte maturation (Figure 3)

This simple sequence of amino acid residues not only has the capability of tissue regeneration and the enhancement of collagen and glycosaminoglycans synthesis but also is able to increase nerve outgrowth and angiogenesis