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The decision depends on individual goals, tolerance, and how much additional weight loss justifies the additional side effect burden

Several factors restrict the number of suitable sites for placement of a large tag such as GFP, Halo or SNAP: (i) the large aforementioned nested N-terminal domains, (ii) the presence of an N-terminal signal peptide that gets cleaved off during polypeptide maturation, (iii) the need to retain the ability to pair with integrin subunits to bind ligand, and (iv) the need to accommodate the large-scale conformational rearrangements thought to occur during integrin activation

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However, systemic copper loading remains a theoretical concern requiring careful dose optimization and copper status monitoring, particularly with chronic administration protocols