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Following the injection, imaging was performed using an IVIS Spectrum system (PerkinElmer) at various time points: 1 h, 4 h, 7 h, 12 h, 24 h, 36 h, 48 h, and 60 h post-injection, with an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm

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Nitrous oxide misuse can cause vitamin B12 deficiency and absorption issues, which can lead to nerve damage, paralysis, chronic numbness, and loss of coordination

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