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Bakirci, S., Dabague, J., Eder, L., McGonagle, D

The following flow antibodies were used: anti-mouse CD4 PE/Cy7 (clone GK1.5) (BioLegend, 100422), anti-mouse IL-17a PE (clone TC11-1810.1) (BioLegend, 506903), anti-mouse CD45 PerCP/Cy5.5 (clone 30-F11) (BioLegend,103131), anti-mouse GATA3 (BioLegend, 653809), antimouse/human CD11b PE/Cy7 (clone M1/70) (BioLegend, 101216), anti-mouse/human CD11b Brilliant Violet 605 (clone M1/70) (BioLegend, 101237), antimouse inducible NO synthase (iNOS) (Invitrogen, 125920), antimouse Foxp3 Pacific blue (clone MF-14) (BioLegend, 26410), antimouse/human Arg1 FITC (R&D Systems, IC5868F), anti-mouse F4/80 PerCP/Cy5.5 (clone BM8) (BioLegend, 123127), antimouse MGL2/CD301B (BioLegend, 146807), antimouse/human T-bet PE/Cy7 (clone 4B10) (Biolegend, 644824), antimouse/human RORt (APC, clone AFKJS-9) (Invitrogen, 17698882), antimouse Ly6G (clone 1A8) (BioLegend, 127613), and PE antimouse CD206 (clone C068C2) (BioLegend, 141705)

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Children and adolescents undergo significant metabolic and physiological changes due to growth and biological maturation that can increase ROS production, which may be accompanied by oxidative stress if levels of endogenous antioxidants are insufficient 14 , 23 , 33