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2000, BRISTOL-MYERS SQUIBB COMPANY, EU, EP 0 646 646 B1 Huang XB, Cyrille D, Thomas P, Bulmus V: Synthesis of Hetertelechelic polymers with affinity to glutathione-s-transferase and biotin-tagged proteins by RAFT polymerization and thiol-ene reactions
Our roGFP2 measurements confirm (i) that dynamic roGFP2 sensing (or the physiological glutathionylation/deglutathionylation of regular surface-exposed cysteinyl residues) requires a glutaredoxin activity, and (ii) that glutathionylated proteins or metabolites should be very transient redox species when GSH and glutaredoxins are present (unless they are stabilized by a kinetic uncoupling mechanism) 5,32,34,35,61,62,63,64
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