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Other groups have explored the concept of GLP-1/GIP/glucagon triagonism to obtain extra benefits from adding the benefits of glucagon action (promotion of energy expenditure and amelioration of fatty liver disease) to the twincretin effects of GLP-1 and GIP
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These results prompted the hypothesis on a direct role of GLP-1 in controlling pubertal development through the hypothalamic GnRH secretion, although this mechanism still remains to be unraveled
Random primers and avian myeloblastosis virus reverse transcriptase were purchased from Promega (Madison, Wisconsin, USA)
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