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The sweet spot for 3mg vials is 1.2mL of bacteriostatic water

PMC 6512700

Hou P-F, Zhu L-J, Chen X-Y, Qiu Z-Q

Rao SV, Kirsch B, Bhatt DL et al (2022) A multicenter, phase 2, randomized, placebo-controlled, double-blind, parallel-group, dose-finding trial of the oral factor XIa inhibitor asundexian to prevent adverse cardiovascular outcomes after acute myocardial infarction

Then a biotinylated detection antibody specific for Rat GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated