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doi: 10.3389/fphar.2023.1267765 Received 27 July 2023 Accepted 09 October 2023 Published 13 November 2023 Volume 14 - 2023 Edited by Xinwang Yang, Kunming Medical University, China Reviewed by Yipeng Wang, Soochow University, China Lin Wei, Anhui Medical University, China Updates Copyright 2023 He, Wang and Qu

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Peptides were separated by chromatography with the following parameters: Acclaim PepMap100 C18 pre-column (2 cm, 75 m i.d., 3 m, 100 ), Pepmap-RSLC Proxeon C18 column (75 cm, 75 m i.d., 2 m, 100 ), 300 nl/min flow rate, using a gradient rising from 95% solvent A (water, 0.1% formic acid) to 40% B (80% acetonitrile, 0.1% formic acid) in 120 min, followed by a column regeneration of 20 min, for a total run of 140 min

In a multi-receptor agonist, however, glucagon receptor activation occurs alongside GLP-1 and sometimes GIP receptor activation