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Never refreeze a reconstituted vial
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Transient blood pressure elevation, typically 5 to 10 mmHg systolic lasting several hours
Isolated islets were transferred to non-adherent dishes containing RPMI-1640 medium with 2 mM L-glutamine and 11 mM D-glucose supplemented with 10% FBS and 1% penicillin/streptomycin (p/s) (P4458, Sigma Aldrich) in a humidified incubator at 37C and 5% CO 2
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