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Protein concentrations were measured with the BCA assay and adjusted to 2 g l in 40 l with the Andrew Plus pipetting robot from Waters

Immune metabolites [13], such as serine [14], succinate [15], -ketoglutarate [16], L-carnitine (LC) [17] and fatty acids (FAs) [18], are capable to manipulate polarization by remodeling intracellular metabolism in macrophages

Ideal candidates are generally healthy and have yet to find sufficient success with traditional weight loss methods

27,28 These signaling markers can be detected with help of robust primers, probes, and antibodies, commercially available across commercial vendors