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The peak occurred at 3 h pi , followed by a continuous decrease until 9 h pi and a subsequent return to the initial level
Affinity K D values were fitted by steady state affinity fitting models (1:1 binding model) using Biacore Insight Evaluation Software, experimental data were analyzed using the Biacore Insight Evaluation Software, with concentration as the X-axis and steady-state response as the Y-axis
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